Characterisation of Phage Susceptibility Variation in Salmonellaenterica Serovar Typhimurium DT104 and DT104b

The surge in mortality and morbidity rates caused by multidrug-resistant (MDR) bacteria prompted a renewal of interest bacteriophages (phages) as clinical therapeutics natural biocontrol agents. Nevertheless, phages are continually under the pressure evolutionary phage–host arms race for survival, which is mediated co-evolving resistance mechanisms. In Anderson phage typing scheme Salmonella Typhimurium, epidemiologically related definitive types, DT104 DT104b, display significantly different susceptibility profiles. This study aimed to characterise mechanisms genomic differences that may be responsible divergent reaction patterns S. Typhimurium DT104b using whole genome sequencing (WGS). analysis intact prophages, restriction–modification systems (RMS), plasmids clustered regularly interspaced short palindromic repeats (CRISPRs), well CRISPR-associated proteins, revealed no unique genetic determinants might explain variation among two types. Moreover, genes coding potential receptors strains. However, findings propose need experimental assessment phage-specific on bacterial cell surface transcriptome RNA will phages. Using bacteria-phage interaction help improving our understanding host–phage interactions ultimately lead development phage-based technologies, enabling effective infection control.